|
KMID : 0358219940210010077
|
|
Korean Journal of Fertility and Sterility
1994 Volume.21 No. 1 p.77 ~ p.82
|
|
|
Improvement of Embryonic Cell development by Coculture with Ampullary cells
|
|
김정호
홍기연/김기석/최정훈/민부기
|
|
|
Abstract
|
|
|
To improve in vitro embryonic cell development, this study was designed to culture in vitro fertilized early embryos of mouse in two different systems; conditioned medium alone and ampullary cells co-culture.
Thirty two of 83 embryos (38.6%) were blocked in the 2 cell stage by co-culture, as compared to forty of 42 embryos (95.2%) in control group for 24hours culture.
And all the embryonic cells cultured for conditioned medium alone were blocked for 48 hours culture.
Twenty seven of 46 embryos (58.7%) which overcome culture block in 2 cel stage by co-cultured were developed morular and expanded blastocyst, and ninteen of 46 embryos (26.1%) underwent hatching for 96 hours culture.
The cellular fragmented rates for embryo were 26.2% in medium alone; 10 fragmented blastomere were graded mild status and 1 fragmented blastomere in severe status.
On the other hand, the fragmented rate for 48 hours co-cultured were 15.7% (13/83); 8 fragmented embryos were graded mild status, moderate status in 3 fragmented embryos and severe in 2 fragmented embryos respectively.
In conclusion, the co-culture of embryos cells is good to improve quality of embryos and overcome of culture block as well as development of cell cleavage.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|